85-1378-08　MssI (PmeI) 1250U　M4692-60

特徴

• 5'-G T T T^A A A C-3' 3'-C A A A^T T T G-5'
• Concentration: 5u/ul
• Source:Methylobacterium species Dd 5–732
• Unit Definition:One unit is defined as the amount of Mssl required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
• Diluent Buffer :10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Storage Buffer:10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
• Supplied with:R1625-Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37°C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
• R1625-04 Restriction Enzyme Buffer E, 10X: Dilute to 1X for 100% Mssl digestion. 10mM Tris-HCl, pH 7.5, 10mM MgCl2 and 0.1mg/ml BSA.
• Incubation Temperature:37°C
• Storage and Stability:Aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.