Murine and human Ang-2 are 496 AA secreted angiogenic proteins. There is ~85% homology between mouse and human Ang-2. It is only 60% homologous with Ang-1. Ang-2 may act as an antagonist of Ang-1 and Tie-2. It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus). This is the control peptide used as the immunogen for Catalog Nos. A2293-10 and A2293-11.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:ELISA: 50-100ng of control peptide/wellAntibody Blocking: 5-10ug per 1ul A2293-10 (antiserum) or per 1ug A2293-11 (affinity purified antibody)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile PBS. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Synthetic peptide
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitution: Reconstitute with 100ul sterile PBS.
Specificity：Synthetic peptide (20aa) near the N-terminus of the mouse Ang-2 (KLH coupled). No significant sequence homology with Ang-1, Ang-3, Ang-4 or ARP’s. Species Sequence Homology: Rat: 100%; porcine: 85%; human: 73%; chicken: 55% chicken.
