The seeds of Griffonia simlicifolia contain four distinct lectins. Two of these lectins, referred to as GS-I and GS-II, have been well characterized with regard to binding specificity and structure. GS-II is specific for terminal, non-reducing alpha or beta linked N-acetyl-D-glucosamine. This lectin is not blood group specific, unlike GS-I. GS-II is also a tetramer. It is composed of identical subunits. Each subunit has a single carbohydrate binding site for GlcNAc. GS-II is the only lectin isolated that is specific for only terminal GIcNAc residue. The subterminal saccharide does play an important role in lectin binding.
Biological Activity:20-30ug/ml is required to agglutinate fresh type B blood cells. Lectin activity against all blood types increases after neuraminidase treatment of cells.
Note: Griffonia simlicifolia (GS-1) exists as a mixture of 5 isolectins. Each isolectin is a tetramer consisting of different amounts of subunits termed A and B. The number after the subunit designation indicates the quantity of each subunit associated with the tetramer form. The GS-1B4 isolectin has four B-subunits. It has a specificiety for type B ethrocytes.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile buffer of choice, aliquot and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Griffonia simlicifolia
Grade：Purified
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.5mM calcium chloride. Reconstitute with buffer of choice. Note: Calcium is required for binding. 0.5mM calcium is the maximum concentration in buffer that will not form a white precipitiate.
