Fyn is a member of the Src family of non-receptor protein tyrosine kinases and is is highly expressed in brain. Fyn has been shown to be involved in T cell and B cell activation and keratinocyte differentiation. Fyn can regulate the activation of PI-3 kinase in CTLL-2 cells, a murine interleukin 2 responsive cell line. In T cells, Fyn associates with the T cell antigen receptor and Thy-1. A study of human T-cells revealed that Lck and Fyn had different intracellular locations, suggesting that these two members of the Src family regulate different aspects of T cell activation. The Src homology 3 (SH3) region of Fyn can bind the proline-rich domain of the Fas ligand. In addition, Raf-1 may form a complex with Fyn through its Src Homology 2 (SH2) domain and serine phosphorylation of Raf-1.
Source:N-Terminal His-Tagged, full-length human Fyn, expressed by baculovirus in Sf21 insect cells.
Molecular Weight:~61.8kD
Specific Activity:1290U/mg
Unit Definition:One unit of Fyn activity is defined as 1nmol phosphate incorporated into 250uM cdc2 peptide/minute at 30°C with a final ATP concentration of 100uM.
Fyn Kinase Assay:~1.7mU-1.7U phosphorylates the Src substrate peptide in vitro.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus in Sf21 insect cells
Grade：Purified
Purity：~70% (SDS-PAGE and densitometry)
Form：Supplied as a liquid in 50mM Tris, pH 7.5, 0.15M sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 0.2mM PMSF, 0.1% beta-mercaptoethanol, 1mM benzamidine, 5% glycerol.
