Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases. The retinoblastoma gene RB was the first tumor suppressor gene cloned, and is a negative regulator of the cell cycle through its ability to bind the transcription factor E2F (MIM 189971) and repress transcription of genes required for S phase (Hanahan and Weinberg, 2000 [PubMed 10647931]).[supplied by OMIM]
Recombinant fusion protein corresponding to the C-terminal fragment (residues 773–928) of human retinoblastoma protein (Rb) with an N-terminal His6-tag, expressed in E. coli.
Application:Suitable for use in Kinase Assay. Other applications not tested.
Recommended Dilution:Kinase Assay: Rb is an effective substrate for a number of serine/threonine kinases. Tested using cdk2/cyclin A, active (C2571-95A) to phosphorylate 10ug Rb.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：500ug
Source Antigen：Recombinant fusion protein containing the C-terminal fragment, expressed in E. coli.
Grade：Highly Purified
Purity：Purified using Ni 2+-NTA agarose. Purity ≥90% (SDS-PAGE and Coomassie blue staining)
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 500mM imidazole.
