85-1376-48　［受注停止］Streptavidin (HRP) 1mg　S7973-97A

特徴

• Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react non-specifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
• Recommended Dilution:ELISA: 1:20,000-1:200,000Western Blot: 1:10,000-1:40,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.