Streptavidin/FITC can be used in conjunction with biotinylated antibodies as an avidin/biotin labeling system for flow cytometry.
Streptavidin (MW ~66kD) binds specifically with biotin (244D). It is derived from the bacterium Streptomyces avidinii and bears a remarkable similarity to chicken egg-white avidin both in three-dimensional structure and its ability to bind biotin with extremely high affinity (Kd=10e-15M). It is a tetrameric protein capable of binding up to 4 biotin molecules. Unlike avidin, Streptavidin is non-glycosylated and is essentially neutral in charge, whereas avidin (pH 10.5) is basic at neutral pH. Because of this, streptavidin has considerably less non-specific binding resulting in less background. It has replaced avidin as the reagent of choice for most applications where protein interactions may cause background.
Application(s):Suitable for use in FLISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Fluorochrome/Protein Ratio:~3.7 moles FITC per mole of Streptavidin
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：S. avidinii
Grade：Highly Purified
Purity：Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against source serum protein and IgG.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide.
