Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Like its namesake avidin, streptavidin binds 4 moles of biotin per mole of protein with a high affinity virtually unmatched in nature (Kd~10-15). Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality where most useful biological interactions occur (pI of 5-6 vs 10 for avidin). As a result, streptavidin frequently exhibits lower levels of non-specific binding than does avidin when the proteins are used in applications relying upon the formation of avidin/biotin complexes (Chaiet and Wolf, 1964).
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:ELISA: 1:100-1:20,000 Assayed against 1ug of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at RT
Western Blot: 1:400-1:2000Immunohistochemistry: 1:200-1:1:1000Optimal dilutions to be determined by researcher.
Storage and Stability:Store at 4° C before opening. DO NOT FREEZE. This product is stable at 4°C as an undiluted liquid. Stable for 6 months after receipt. Dilute only prior to immediate use. Freezing Beta Galactosidase conjugates will result in a substantial loss of enzymatic activity.
仕様
Size：1mg
Grade：Highly Purified
Purity：Purified by ion exchange chromatography. Assay by IEP demonstrated a single precipitin arc against anti-beta galactosidase (E. coli) and anti-streptavidin. No crossreactivity was observed against anti-avidin.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01M magnesium chloride, 0.01M Beta-Mercaptoethanol, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeld with Beta Galactosidase (E.coli)
