Applications:Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:1mg
Source Antigen:Sheep
Grade:Highly Purified
Purity:Purified by ion exchange chromatography.
Form:Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).Reconstitution: Reconstitute with 1ml sterile distilled water or 40-50% glycerol.
Specificity:IEP Assay shows a single precipitin arc against anti-peroxidase, anti-sheep IgG, anti-sheep IgG Fc, and anti-sheep serum. Does not crossreact with anti-sheep IgG F(ab’)2 or anti-papain.