Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG Fab molecule
Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and immunomicroscopy. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Rabbit
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography, followed by pepsin digestion and dialysis.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), and 0.01% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Specificity：IEP Assay shows a single precipitin arc against anti-biotin, anti-rabbit IgG, anti-rabbit IgG F(ab’)2 and anti-rabbit serum. No reaction observed against anti-rabbit IgG F(c) or anti-Pepsin.
