Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Application:Chicken IgG F(ab')2 fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Storage:Store vial at 4°C prior to opening. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
仕様
Size：2mg
Source Antigen：Chicken
Grade：Highly Purified
Purity：Prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken IgG F(ab’)2 and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(c) or anti-Pepsin.
