Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Applications:Suitable for use as a control or standard in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:100ug
Source Antigen:Human adult knee cartilage
Grade:Highly Purified
Purity:Purified by ion exchange chromatography. Free from other collagens, human serum proteins, and non-collagen extracellular matrix proteins
Form:Supplied as a liquid in 0.5M acetic acid, 0.01% sodium azide. No stabilizing proteins added.
Specificity:Reaction with anti-Collagen I, III, IV, V or VI is negligible (typically less than 1% crossreactivity was detected by ELISA)