Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and glucoseoxidase-antibody based enzymatic assays. Other applications not tested.
Recommended Dilutions:ELISA: 1:8000-1:32,000Western Blot: 1:500-1:2500Immunohistochemistry: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:2mg
Source Antigen:Hen egg white
Grade:Highly Purified
Purity:Prepared from chromatographically pure avidin followed by dialysis.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with glucose oxidase from Aspergillus niger. Glucose oxidase (GO) MW - 160kD. Reconstitute with 1ml sterile ddH2O.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-avidin and anti-glucose oxidase.