Purity:Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against source serum protein and IgG.
Appearance:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml PEG-8000, 0.01% sodium azide. Labeled with TRITC (444D). Reconstitute with 1ml ddH2O.
Concentration:~1mg/ml (after reconstitution)
Specificity:A single precipitin arc is produced in immunoelectrophoresis using this protein against anti-bovine albumin and anti-bovine serum.
Applications:Suitable for use in FLISA, Fluorescent Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilutions: Optimal dilutions to be determined by the researcher.
Fluorochrome/Protein Ratio:4.0 moles TRITC per mole of bovine albumin
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Light sensitive
