85-1373-49　Bpl I 100U　B2645

特徴

• 5'-^ 8(N) G A G (N)5 C T C (N)13^-3'3'-^13(N) C T C (N)5 G A G (N) 8^-5'
• BplI requires only Mg2+ for its activity, but is stimulated by S-adenosylmethionine. 0.05mM S-adenosylmethionine gives more than a 100-fold increase of BplI activity. The cleavage of DNA by BplI is never complete.
• Concentration:5 units/ul
• Unit Definition:One unit is defined as the amount of Bpl I required to digest 1ug of lambda DNA-Xhol in 1 hour at 37°C in 50ul of assay buffer (a maximal cleavage level is achieved at which no change in the fragmentation pattern is observed with further increase of enzyme).
• Storage Buffer:Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Buffers Supplied:R1625 Restriction Enzyme Buffer A, 10X:33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA B2645-01: SAM0.05mM S-adenosylmethionine
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.