85-1373-08　Ro/SS-A Antigen (Ro-60/SS-A) 100u　R2997-04

特徴

• The (Ro) SS-A antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases (1). The antigenic activity of the (Ro) SS-A antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA (1). Anti-(Ro) SS-A autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome and Systemic Lupus Erythematosus patients. The presence of autoantibodies against the (Ro)SS-A antigen may be used as a diagnostic aid. Elevated levels of Anti-(Ro) SS-A have been detected in as high as 96% of Sjogren's Syndrome patients (2), 49% of patients with Systemic Lupus Erythematosus (2), 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus. Recently, a strong correlation between low positive levels of Anti-(Ro) SS-A and subdinical dry eyes or mouth was found in a substantial portion of an elderly population (3). In addition, it has been shown that 70% of patients with (Ro) SS-A precipitating antibodies also have rheumatoid factor (4). The titer of rheumatoid factor and the concentration of Anti-(Ro) SSA have been closely correlated in Primary Sjogren's Syndrome (4). Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers.
• Activity:1000u/ml
• Unit Definition:One unit of activity is the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for ELISA. Activity will vary with assay conditions/ protocols.
• Applications:Suitable for use in ELISA. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by researcher.
• Storage and Stability:Aliquot and store at -70+/-10°C. Aliquots are stable for 6 months after receipt at 70+/-10°C. Avoid freeze-thaw cycles. Note: Product is unstable above 0°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Also Available:R2997: Ro/SS-A Antigen Pab Hu xHuR2997-04: Ro/SS-A Antigen