85-1372-72　Lysozyme, Human Neutrophil (Muramidase) 1mg　L9200-14

特徴

• Lysozyme (muramidase) hydrolyzes preferentially the beta-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. A somewhat more limited activity is exhibited towards chitin oligomers.
• Lysozyme is of widespread distribution in animals and plants. That which has been most extensively studied is from hen egg white (lysozyme C). Lysozyme is also found in mammalian secretions and tissues, saliva, tears, milk, cervical mucus, leukocytes, kidneys, etc.
• Useful for the rapid isolation of RNA from gram negative bacteria. Can be used to prepare small amounts of plasmid DNA.
• Form:Lyophilized from 0.05M sodium acetate, pH 6.0, 0.1M sodium chloride. Reconstitute with ~500ul ddH20.
• Purity:≥ 95% by SDS-PAGE
• Activity:≥30,000 Shugar units per mg.
• Unit Definition:One unit is defined as the amount of enzyme that will digest powdered cells of Micrococcus lysodeikticus, causing a decrease in absorbancy of 0.001 per minute at 37°C, pH 7.0.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.