Cathepsins are a group of intracellular proteolytic enzymes located within the lysosomes. On the basis of the specificity towards their substrates, the cathepsins have been assigned the names: Cathepsins A, B, C, D, E, G H and L. Cathepsin G is a serine protease, it consists of a single polypeptide with a MW of 30kD as determined by SDS-PAGE. Its substrates are collagen and proteoglycans. Cathepsins are localized primarily in lysosomes or endosomes, but translocated to the cytosol during apoptosis. The translocation allows these proteases to interact with caspases or other apoptosis signaling mechanisms. Studies show that cathepsins cleave certain caspases, and caspase inhibitors can inhibit cathepsin activity in vitro.
Unit Definition:One unit of activity is the amount of enzyme that hydrolyzes one micromole of Suc-ala-ala-pro-phe-pNA (1mM) per minute at 25°C in 160mM Tris HCl, pH 7.4, 1.6M sodium chloride.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human Neutrophil
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder. No preservatives added. Reconstitute with 50mM sodium acetate, pH 5.5, 150mM sodium chloride as the buffer.
