Alpha amylase is able to catalyze the hydrolysis of starch (amylose and amylopectin) to a significant extent resulting in the release of maltose. The enzyme is found in nearly all plants, animals and microorganisms. The enzymes from various sources exhibit marked differences in physical, chemical and catalytic properties. Bacterial amylases and those from porcine pancreas and human saliva have been extensively studied. Alpha amylase is widely used in the food industry as a clarifying agent. Determination of serum alpha amylase activity, for the diagnosis of acute pancreatitis, is a widely used procedure in clinical medicine (Brooks, F., New Eng. J. Med., 286, 300, 1972). Additionally, it has been reported that elevated alpha amylase activity in serum also occurs in mumps, renal disease and abdominal disorders such as cholecystitis (Salt, W. and Schenkder, S., Medicine, 55, 269, 1978).
E.C. No:3.2.1.1
Gene:AMY2A, AMY2, AMY2B, PA
Gene ID:279
Accession No:P04746
Source:Human Pancreas
Concentration:~2mg/ml Protein
Appearance:Supplied as a liquid in 100mM Tris-HCl, 150mM sodium chloride, 1mM calcium chloride, 1mM benzamidine, 1mM pefabloc, pH 7.5. No preservatives added.
Unit Definition:One unit will catalyze the hydrolysis of one micromole of 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100U
Source Antigen：Human Pancreas
Grade：Highly Purified
Purity：Purified
Form：Supplied as a clear, colorless to light yellow liquid in 100mM Tris-chloride, 150mM sodium chloride, 1mM calcium chloride, pH 7.5. No preservatives added.
