Control Peptide for S9500-21(antiserum) and S9500-21A (affinity purified). 15 aa peptide located near the C-terminus of human synuclein-alpha. Corresponding antibody recognizes a-Synuclein CT. No significant homology is seen with beta-synuclein or with other known proteins.
Species homology: Rat, mouse and chicken: 86%
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. An early step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid1-40, 1-42, and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140aa a-synucleins is identical with non-Ab component (NACP) of AD. The 134aa b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine has the potential to cause the protein to misfold.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:ELISA: 50-100ng of control peptide/wellAntibody Blocking: 5-10ugOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Grade：Highly Purified
Purity：Highly purified
Form：Suppled as a lyophilized powder in PBS, pH 7.5, 0.05% sodium azide.
