85-1371-83　Fibroblast Cell, 3T3/A31 50ul　F4209

特徴

• Cellular protein preparation from murine 3T3 fibroblasts.
• Applications:Suitable for use in Western Blot as a Positive Control. Other applications not tested.
• Recommended Usage:Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Immunoblot analysis
• Optimal dilution to be determined by researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

仕様

• Size：50ul
• Source Antigen：Murine cells
• Grade：Lysate
• Form：Supplied as a liquid in modified RIPA buffer with non-reducing sample buffer. Cellular Protein Preparation: Cells were lysed in modified RIPA buffer (50mM Tris HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM sodium vanadate, 1mM sodium fluoride). Diluted with non-reducing sample buffer (31mM Tris HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromophenol blue).
• Calc Applications Abbrev：WB
• EU Commodity Code：38220090
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