Cellular protein preparation from A431 cells containing phosphorylated proteins, including the EGF receptor.
Recommended Dilutions:Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for immunoblot analysis. This preparation may be used as a positive control for antibodies.
Storage and Stability:For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt at -20°C after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Source Antigen：Human A431 cells
Grade：Lysate
Form：A431 cells were cultured for 20 minutes in the presence of 50ng/ml EGF. Cells were lysed in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM Na3VO4, 1mM NaF) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromophenol blue).
