Hyper Broth™ is a proprietary medium formulation for improved expression of recombinant proteins in E. coli. The medium is a complex, rich formulation which supplies amino acids, vitamins, and glucose at high levels. It is made without the use of animal-derived raw materials and is APF Certified. The medium is buffered at pH 7.3 ± 0.2, which permits high glucose levels.
For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 4-5 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Plasmid copy numbers are higher than for LB, which can facilitate protein expression. This medium is suitable as the base for fermentation cultures and is excellent for feed-batch applications. Biomass yields will depend on the process and the strain employed. Hyper Broth™ powder is supplied with P9102-40F: Protein Expression Media, Glucose-Nutrient Mix, M9Y (Powder) pEX™
Appearance:Light to medium brown, homogenous, free flowing powder
Solubility (44.5g/L):Tan to amber, complete
pH:7.3 ± 0.3
Also Available:P9102-40D: Protein Expression Media, HyperBroth™ (Powder) pEX™P9102-40D1: Protein Expression Media, HyperBroth™ (Liquid) pEX™
