5'.....G A G^C T C.....3' 3'.....C T C^G A G.....5'
Unlike SacI, Ecl136II produces DNA fragments with blunt ends.
Source: Enterobacter cloacae RFL136
Concentration: 10 units/ul
Unit Definition:One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-HindIII fragments in 1 hour at 37°C in 50ul of assay buffer.
Supplied with:R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA (pH 7.9 at 37ºC).
Supplied with:R1625-55: Restriction Enzyme Buffer for Ecl136II, 10X: Supplied as a liquid 10mM Bis-Tris Propane-HCl, pH 6.5, 10mM MgCl2, 0.1mg/ml BSA. Incubate at 37°C.
Diluent and Storage Buffer:10mM Tris-HCl , pH 7.4 at 25°C, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
