Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa, SHPS-1, MyD-1, BIT and p84. SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion. It plays a general role in different physiological and pathological processes.
Applications:Sutiable for use in Antibody Blocking. Other applications not tested.
Recommended Dilutions:Antibody Blocking: Use equal volume of S1013-87A with S1013-87. Incubate for 30 minutes at 37°C
Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Human SIRP alpha1, aa487-503
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.
Specificity：Synthetic peptide corresponding to aa487-503, KPEPSFSEYASVQVPRK of human SIRPa1. The sequences of immunogenic peptide differ from those of mouse, rat and bovine by one amino acid.
