Peptide corresponding to aa525-540 of human IL-1RAcP, which is identical to those of mouse and rat origins The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kB inducing kinae (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP (2). IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP (3,4). Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.
Applications:Suitable for use as blocking peptide in Western Blot with I5000-05.
Recommended Dilution:Blocking: Incubation of I5000-05P with equal volume of I5000-05 for 30 min. at 37°C typically blocks antibody activity in Western Blot completely.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Synthetic peptide
Grade：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.
