Peptide corresponding to amino acids 313 to 331 of human DFF45 (1). Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45kD DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis (1). Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2-5). Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
