Synthetic peptides, 16 aa, near the N-terminus of rat Orexin 1 Receptor (Hypocretin 1 Receptor).
Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus and are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis. These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs). Rat and human Orexin 1Receptor (Hypocretin 1 Receptor) are 416 aa, and 425aa, respectively.
Applications:Suitable for use in ELISA or antibody blocking to confirm antibody specificity. Not suitable for Western Blot due to low MW (<3kD). Other applications not tested.
Reccommended Dilutions:ELISA:Coat with 50-100ng Control Peptide/well
Antibody blocking:Use 5-10ug per 1ug of O7503-05 affinity purifiedor 1ul of O7503 SerumOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Rat synthetic peptide
Grade：Highly Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Species Crossreactivity: No crossreactivity with OX2R or other G-protein-coupled receptors.Species sequence homology: Mouse: 100%, Human 82%
