Control Peptide for O7502 (antiserum) and O7502-05 (affinity purified)
Synthetic peptide, 13aa, murine Orexin A Pre-pro immunogenic peptide shows 82% (14/17 aa) and 70% (12/17 aa) identity with rat and human prepro-orexins, respectively. The sequence is not found in Orexin A or Orexin B. Therefore, antibodies will not detect mature Orexin A or Orexin B. Use the appropriate control immunogenic peptides to confirm specificity of antibodies.
Hypothalamic neuropeptides associated with feeding behavior, binding and activating two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R. Recognizes the full-length (1-33 aa) Orexin-A. The epitope is not found in Orexin B. Since Orexin A and Orexin B are derived from an unprocessed prepro-orexin (130 aa), anti-Orexin A antibodies may also react with pre-pro orexins.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended Dilution:ELISA: 50-100ng control peptide/well
Antibody Blocking: 5-10ug per 1ug O7502-05 (affinity purified antibody) or per 1ul O7502 (antiserum)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Grade：Highly Purified
Purity：Chromatographically purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
