Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12kD mature glycoprotein consisting of 109 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor beta superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.
Human Myostatin is created as a recombinant protein with N-terminal fusion of HisTag with added 43 amino acid sequence as a spacer. The Human Myostatin-HisTag, produced in E. coli, is 16.7kD protein containing 109 amino acid residues, aa267-375, of human Myostatin and 43 additional amino acid residues for a total of 152aa.
Human Myostatin is recombinant protein with N-terminal fusion of HisTag with added 43 amino acid sequence as a spacer: Met-Arg-Gly-Ser-His-His-His- His-His-His-Gly-Met-Ala-Ser-Met-Thr-Gly-Gly-Gln-Gln-Met-Gly-Arg-Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys-Asp-Pro-Ser-Ser-Arg-Ser-Ala-Val-Arg-Ser-Arg-Arg. Swiss/UniProt Accession: O14793
Applications:Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with acetate buffer. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Human, recombinant (E. coli).
Grade：Highly Purified
Purity：≥95% by SDS-PAGE. Endotoxin: ≤1EU/ug
Form：Supplied as a lyophilized powder from 0.05M acetate buffer, pH 4.0. Reconstitution: Reconstitute with 0.2ml of sterile 0.1M acetate buffer, pH 4. Allow the lyophilized pellet to dissolve completely. For conversion into higher pH value, intensive dilution by relevant buffer to 10ug/ml is recommended. In higher concentrations the solubility of this antigen is limited.
