Nuclear extract is prepared from human HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins and transcription factors.
Applications:Suitable for use as a positive control for antibodies.
Recommended Usage:Add an equal volume of Laemmli reducing sample buffer to 5-10ul of lysate. Boil for 5 minutes to reduce the preparation. Load 5-10ul (10-20ug) of reduced lysate per lane for immunoblot analysis.
Storage and Stability:For long-term storage, store at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：HeLa cells
Grade：Lysate
Form：Supplied as a liquid in RIPA buffer containing inhibitors.
