85-1369-29　MAPKAP Kinase 2, Active, Recombinant, Human (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2) 10ug　M2355-05

特徴

• Recombinant human MAPKAP Kinase 2, residues 46-400, A399G, containing an N-terminal GST tag; expressed in E. coli.
• Specific Activity:~2000U/mg.
• Unit Definition:1 unit of MAPKAP kinase 2 activity is defined as 1nmol phosphate incorporated into 30uM substrate peptide (KKLNRTLSVA), (Cat #M2355-09) per minute at 30°C with a final ATP concentration of 100uM.
• Kinase Assay:2.2-6ng of enzyme phosphorylated 30uM MAPKAP kinase 2 substrate peptide
• MS Tryptic Fingerprint:Confirmed identity as MAPKAP kinase 2 with 23% amino acid coverage of the translated native sequence (see protocol).
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

仕様

• Size：10ug
• Source Antigen：Recombinant, E. coli
• Grade：Purified
• Purity：≥90%
• Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.1% 2- mercaptoethanol, 0.02% Brij-35, 50% glycerol.
• Molecular Weight Non Ab：70.2
• Swiss Prot Number：P49137
• EU Commodity Code：30021019
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• 製品の仕様は予告なく変更になる場合がございます。最新仕様はメーカーホームページをご確認ください。

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