p38-regulated/activated protein kinase (PRAK) is a 471 amino acid protein with 20–30% sequence identity to the known MAP kinase-regulated protein kinases RSK1/2/3, MNK1/2, and MAPKAP-K2/3. PRAK is activated by stress-related extra-cellular stimuli. The regulatory phosphorylation site of PRAK is Thr182, which is phosphorylated by p38 alpha and p38 beta in vitro. Activated PRAK specifically phosphorylates HSP27 at its physiologically relevant sites in vitro.
Recombinant protein corresponding to full-length human PRAK, fused with an N-terminal His-Tag, expressed in Sf9 cells.
Molecular Weight:~54kD
Specific Activity:~3.5units/mg after activation with SAPK2a/p38 a.
Unit Definition:One unit of PRAK activity is defined as 1nmol phosphate incorporated into substrate peptide per minute.
PRAK Activation Assay:Unactive PRAK, after activation with 0.06Units of SAPK2a/p38a, phosphorylated PRAK Substrate Peptide (KKLRRTLSVA) 13.5-fold over background
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, human from Sf9 cells
Grade：Purified
Purity：~85%. Purified using Ni-NTA agarose.
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 0.15M sodium chloride, 0.1mM EGTA, 0.1% 2- mercaptoethanol, 0.02% Brij-35, 50% glycerol.
