p38-regulated/activated protein kinase (PRAK) is a 471 amino acid protein with 20–30% sequence identity to the known MAP kinase-regulated protein kinases RSK1/2/3, MNK1/2, and MAPKAP-K2/3. PRAK is activated by stress-related extra-cellular stimuli. The regulatory phosphorylation site of PRAK is Thr 182 ; PRAK is phosphorylated by p38 a and p38 ß in vitro at this residue. Activated PRAK specifically phosphorylates HSP27 at its physiologically relevant sites in vitro.
Recombinant protein corresponding to the full-length amino acid sequence of human PRAK, fused to an N-terminal 6X His-Tag, expressed in Sf21 cells.
Molecular Weight:~54kD
Specific Activity:~30units/mg.
Unit Definition:One unit of PRAK activity is equal to 1nmol phosphate incorporated into substrate peptide in one minute.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：15ug
Source Antigen：Recombinant, Baculovirus in Sf21 insect cells
Grade：Purified
Purity：≥90%. Purified using Ni-NTA agarose, activation by MAP Kinase and removal of activator on GSH-agarose.
Form：Supplied as a liquid in 50mM Tris- HCl, pH 7.5, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.15M sodium chloride, 0.27M sucrose, 0.02% Brij-35.
