This gene encodes a serine/threonine protein kinase that is highly similar to the rat serum-and glucocorticoid-induced protein kinase (SGK). This gene was identified in a screen of hepatocellular genes regulated in response to cellular hydration or swelling. Cellular hydration is a catabolic signal, stimulating glycogenolysis and proteolysis, and inhibiting protein and glycogen synthesis. This kinase has been shown to be important in activating certain potassium, sodium, and chloride channels. Expression of this gene in hepatocytes is stimulated by transforming growth factor-beta (TGF-beta) which participates in the pathophysiology of diabetic complications. Since both TGF-beta expression and SGK expression are elevated in diabetic nephropathy, this suggests an involvement of SGK in the development of this condition.
Source:Human SGK1 fusion protein containing a S422D mutation, N-truncated (missing N-terminal 59aa), fused to N-terminal 6X His-Tag, expressed in Sf21 cells. Upon phosphorylation by PDK1 on T256, this protein becomes fully activated.
Specific Activity:~14300U/mg after activation with PDK1.
Unit Definition:One unit of SGK (S422D) activity is defined as 1nmol phosphate incorporated into Crosstide in one minute.
Quality Control:Activation Assay:Unactive SGK (S422D) was activated using PDK1 (cat# P3123-01) and the increased activity against crosstide determined. The activation and subsequent assay is described in the protocol.
MS Tryptic Fingerprint:Confirmed identity as SGK (S422D) with the translated sequence.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Source Antigen：Sf21 cells
Grade：Highly Purified
Purity：Purified using Ni-NTA agarose (≥95%).
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 270mM sucrose.
