Jun Kinases (JNK), also called stress-activated protein (SAP) kinases, are proline-directed, serine/threonine- specific kinases that require phosphorylation on tyrosine for phosphotransferase activity. JNK was originally shown to phosphorylate microtubule-associated protein-2, but c-Jun and ATF2 appears to be an important physiological target of this kinase. Phosphorylation of Thr69 and Thr71 of ATF2 by JNK is important for the transcriptional activity of ATF2. cDNA cloning studies with rat and human libraries have shown that there are several isoforms of the JNK family. Many stimuli activate JNK including heat shock, hyperosmotic shock, UV irradiation, exposure to protein synthesis inhibitors such as cycloheximide and anisomycin, and treatment with proinflammatory cytokines such as tumor necrosis factor-a.
Recombinant full-length protein corresponding to human JNK1a1/SAPK1c with an N-terminal His-tag, expressed in Sf21 cells.
Specific Activity:< 1% of maximum activity. Activated by phosphorylation with MKK4 and MKK7beta1.
JNK1a1/SAPK1c Kinase Assay:2uM of unactive JNK1a1/SAPK1c was activated using 0.1uM MKK4 and 0.1uM MKK7b1.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant human
Grade：Highly Purified
Purity：Purified using Ni-NTA agarose. Purity ≥90%. Mr ~45kD. Purity was assessed by SDS-PAGE and Coomassie blue staining of 3ug of unactive JNK1a1.
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.02% Brij-35, 50% glycerol.
