Recombinant protein corresponding to full-length human JNK1a1/SAPK1c with an N-terminal His-tag, expressed in Sf21 cells.
Specific Activity:39u/mg where one Unit of JNK1a1/SPAK1c activity is equal to 1nmol phosphate incorporated into GST-ATF2 per minute.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:10ug
Source Antigen:Recombinant, Sf21 cells
Grade:Purified
Purity:≥90% using Ni-NTA agarose. Mr ~43kD. Purity was assessed by SDS-PAGE and Coomassie blue staining using 2.5ug of active JNK1a1.
Form:Supplied as a frozen solution in 50mM Tris-HCl, pH 7.5, 270mM sucrose, 150mM NaCl, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF.