A direct link exists between hyperacetylation of chromatin and transcriptional activation. Experiments have established that PCAF possesses intrinsic histone acetylase activity. PCAF primarily acetylates lysine 14 of H3. It acetylates lysine 8 of H4 less efficiently. PCAF also acetylates p53 in response to DNA damage. The distinct patterns of acetylation by PCAF may contribute to transcriptional regulation of important genes.
Source:Expressed in E. coli corresponding to aa352-832.
Quality Control:HAT Assay: 500ng of PCAF was used to acetylate histones in vitro with tracer levels of [3H]AcetylCoA as the acetyl donor.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：E. coli
Grade：Purified
Purity：~30%
Form：Supplied as a liquid 50mM Tris-HCl, pH 8.0, 150mM sodium chloride, 25mM glutathione, 50% glycerol.
