Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. The enzyme is the mammalian homolog of the zeste-white3 (shaggy) homeotic gene of Drosophila melanogaster and has been implicated in the regulation of the c-Jun/AP-1 transcription factor. In mammals, this protein serine kinase is encoded by two related genes called GSK-3a (51kD) and b (46kD). Genetic and biochemical evidence now indicates that GSK-3 plays a role in intracellular signaling pathways. For example, during insulin and growth factor signaling the GSK-3 beta isoform becomes phosphorylated on its N-terminal serine-9 residue, presumably by either the action of MAPKAP Kinase-1 (p90 S6 kinase, Rsk-2) or p70 S6 kinase. Such phosphorylation of GSK-3 blocks its ability to phosphorylate c-Jun, protein phosphatase inhibitor 2 or the GSK-3 substrate peptide.
Recombinant protein corresponding to human Glycogen Synthase Kinase 3 beta, fused to 6X His-Tag at N-terminal, expressed in Baculovirus (Sf21 insect cells)
Quality Control:MS Tryptic Fingerprint: Confirmed identity as GSK3b with 52% amino acid coverage of the translated native sequence listed in the protocol
GSK3b Kinase Assay: 2-6ng phosphorylated 20uM Glycogen Synthase Peptide 2, Phosphorylated (Substrate) (G8170-26) in the assay described in protocol.
Specific Activity:~900u/mg.
Unit Definition:One unit equals 1nmole phosphate incorporated into 20uM Glycogen Synthase Peptide 2, Phosphorylated (Substrate) (G8170-26) per minute at 30°C with a final ATP concentration of 100uM.
Handling Recommendations:Rapidly thaw the vial under cold water. Immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes. Immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, Baculovirus in Sf21 insect cells
Grade：Purified
Purity：Purified by Ni/NTA agarose chromatography (≥85%, Verified by SDS-PAGE and Coomassie Blue stain).
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol.
