Essentially identical proteins in mammals (p38 MAP Kinases) and yeast (HOG-1). Inhibitors include protein phosphatase 2A, PTP-1B, CL-100 and pyridinyl imidazols.
Activated using a constitutively active mutant of MKK6 and repurified using glutathione sepharose.
Recombinant protein corresponding to human SAPK2b, fused to GST-Tag at N-terminal, expressed in E. coli.
Molecular Weight:~65kD
Specific Activity:143U/mg
Unit Definition:One unit of SAPK2b activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30°C with a final ATP concentration of 100uM.
Applications:Coupled Kinase Assay: Enzyme was tested in vitro in a coupled kinase phosphorylation assay using 20-100ng of active SAPK2b to activate GST-MAPKAP Kinase-2, unactivated as shown by phosphorylation of MAPKAP Kinase-2 Substrate Peptide.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥97.2% (SDS-PAGE and Coomassie blue staining)
Form：Supplied as a liquid in 25ul of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 50% glycerol.
