The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
N-terminal GST-tagged, recombinant human full-length p38 alpha/SAPK2a, expressed in E. coli.
Specificity:Essentially identical in mammals (p38 MAPK) and yeast (HOG-1). Inhibitors include protein phosphatase 2A, PTP-1B, CL-100 and pyridinyl imidazols.
Specific Activity:≥50units/mg using MBP as a substrate.
Unit Definition:One unit of SAPK2a/p38a = 1 nmol phosphate incorporated into MBP/minute.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months after receipt For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Rrecombinant, E. coli
Grade：Highly Purified
Purity：≥93% (SDS-PAGE and Coomassie blue staining)
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 50% glycerol.
