Recombinant GST fusion protein fused to full length human SAPK4, expressed in E. coli.
Human stress-activated protein kinase-4 (SAPK4) is a novel MAP Kinase family member whose amino acid sequence is 60% identical to that of the other three SAP Kinases which contain a TGY motif in their activation domain. Recent results suggest that cellular functions attributed to SAPK1 and/or SAPK2 may be mediated by either SAPK3 or SAPK4.
Specific Activity:~100U/mg where 1unit pf p38 delta/SAPK4 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein/minute at 30°C with a final ATP of 100uM.
Kinase Assay:Enzyme was tested in a functional kinase assay by using 10-100ng of active SAPK4 to phosphorylate MBP in vitro.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Human recombinant expressed in E. coli(full length).
Grade：Affinity Purified
Purity：Purified to ≥50% by affinity chromatography on glutathione Sepharose beads after activation with SKK3/MEK6.
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol, and 50% glycerol.
