An important convergence point involved in the signal transduction pathways of many different growth factors, hormones, and cytokines is a family of 41–44kD serine/threonine kinases collectively called either MAPKs (for mitogen-activated protein kinases) or Erks (for extracellular-regulated kinases). MAPK is activated by sequential phosphorylation on both tyrosine and threonine residues by the dual tyrosine/threonine kinase MEK (MAPK kinase). The serine/threonine kinase Raf can phosphorylate and activate MEK, in turn, during intracellular signaling. Activation of MAPK is directly regulated by a specific MAPK phosphatase and indirectly regulated by protein kinase A activation which results in inhibition of Raf activity in mammalian cells.
SourceUnactive, recombinant full-length mouse p42 MAP Kinase 2/Erk2 fused to GST-Tag at N-terminus, expressed in E.coli.
Specific Activity:The enzyme preparation demonstrates no endogenous kinase activity. This product demonstrates <1% of maximum activity. Activated by phosphorylation with MEK1
Activation Assay:2uM unactive MAP Kinase 2 was activated using 0.4uM active MEK1, diluted 100–fold, and the increased activity against MBP determined. The activation and assay are described in the Protocol section.
MS Tryptic Fingerprint:Confirmed identity as MAP Kinase 2 with the translated native sequence listed in the Protocol section.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥99.5% as determined by SDS-PAGE and Coomassie blue staining. Purified by glutathione-sepharose chromatography.
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2- mercaptoethanol, 1mM benzamidine, 0.2mM PMSF and 50% glycerol.
