Fully active CK-2; heterotetramer of 48.7kD a-subunit and 52.6kD b-subunit (a2b2).
N-terminal 6His-tagged full length human CKa subunit and N-terminal GST-tagged full-length human CKb subunit. expressed individually by baculovirus in Sf21 insect cells. The CKa subunit was purified using Ni2+/NTA-agarose, the b subunit using glutathioneagarose.
Applications:Suitable for use in Kinase Assays and MS Tryptic Fingerprint. Other applications not tested.
Specific Activity:~2000u/mg
Unit Definition; 1unit of human Casein Kinase 2ab, active acitivity is 1nmol phosphate incorporated into 165uM casein kinase 2 substrate peptide (RRRDDDSDDD) per minute at 30°C with a final ATP concentration of 100uM.
Storage and Stability:For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：2x5ug
Source Antigen：Recombinant human protein expressed in Sf9 insect cells.
Grade：Highly Purified
Purity：~80%, Purified by Ni2+/NTA-agarose, the b subunit using glutathioneagarose.
Form：Supplied as a liquid in 20mM MOPS, pH 7.0, 1mM EDTA, 0.1mM Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol.
