Suitable as a substrate for active MAPKAP Kinase-2 and active GST-MAPKAP Kinase-2. It can also serve as a substrate for unactive GST-MAPKAP Kinase-2 upon the enzyme’s activation. The peptide can also be used as a substrate for p90 S6 Kinase and CaM Kinase II. Peptide (KKLNRTLSVA) related to human glycogen synthase residues 1–9.
Kinase Assay:Use 250mM per assay. Tested using 0.5U active MAPKAP Kinase-2 to phosphorylate the substrate peptide.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
