Native Protein G is a surface receptor from Streptococcus sp. which binds the Fc portion of immunoglobulins, binding 2 molecules of IgG.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000-1:200,000Western Blot: 1:10,000-1:40,000Immunohistochemistry: 1:1000-1:5000Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Source Antigen：Recombinant
Grade：Highly Purified
Purity：Purified by Protein G affinity chromatography. Single band by Immunoelectrophoresis.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Gentamicin sulfate. Labeled with Horseradish peroxidase (HRP).Reconstitute with 1ml sterile ddH2O.
