85-1365-63　Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/CaCl2, MgCl2 (Powder) 25L　D9821

特徴

• Dulbecco's Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromotographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process.
• Also Available:D9819: Dulbecco’s Phosphate Buffered Saline (PBS) (Powder)D9820: Dulbecco’s Phosphate Buffered Saline (PBS) w/o CaCl2, MgCl2 (Powder)D9820-04: Dulbecco’s Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder)Dulbecco’s Phosphate Buffered Saline 10X (PBS) w/o CaCl2, MgCl2 (Powder)D9821: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) (Powder)D9822: Dulbecco’s Phosphate Buffered Saline (PBS) w/EDTA, w/o CaCl2, MgCl2 (Powder)D9823: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/MgSO4 (Powder)
• Appearance: White, homogeneous, free flowing powder
• Solubility (1X): Colorless, clear, complete
• Some precipitate may be observed as a 10X due to excess concentration of salts. Sterile filter after solubilization.
• pH: As Reported
• Endotoxin: ≤1EU/ml
• Formulation:Componentsg/LCalcium Chloride•2H2O1.325Magnesium Chloride•6H2O1.00Potassium Chloride2.00Potassium Phosphate Mono2.00Sodium Chloride 80.00Sodium Phosphate Dibasic11.50Total: 97.8g/L
• Directions:To make a 10X solution:Dissolve 97.8g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers..
• Note: Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization.
• USB recommends the preparation of this product as a 1X solution.
• To make a 1X solution:Dissolve 9.78g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers.
• Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
• Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.