85-1365-03　［取扱停止］2’-Deoxynucleotide Triphosphates (Set of 4 dNTP’s) dATP, dCTP, dGTP, dTTP Solution, BioGenomics™ 4x0.5ml/100mM　D3191

特徴

• Suitable for use in PCR™, cDNA synthesis, DNA sequencing and labeling procedures
• Supplied as a set of 4 dNTP’s at a concentration of 100mM
• Functionally tested in PCR with Taq and Pfu DNA Polymerases.
• Components:D3205:2’-Deoxythymidine-5’-Triphosphate, Sodium Salt (dTTP) Solution:Purity (HPLC): ≥99% Concentration: 100mM ± 5%pH: 7.0 ± 0.1Lambda Max: 260nmExtinction Coefficient: 9600 (pH 7, 20mM potassium phosphate) A250/A260: 0.65 ± 0.03A280/A260: 0.73 ± 0.03DNase: None DetectedRNase: None Detected
• D3165:2’-Deoxyadenosine-5’-Triphosphate, Sodium Salt Solution (dATP):Purity (HPLC): ≥ 99% Concentration: 100mM ± 5%pH: 7.0 ± 0.1Lambda Max: 259nm ± 2nmExtinction Coefficient: 15200 (pH 7, 20mM potassium phosphate) A250/A260: 0.80 ± 0.03A280/A260: 0.15 ± 0.03DNase: None DetectedRNase: None Detected
• D3187:2’-Deoxycytidine-5’-Triphosphate, Sodium Salt (dCTP) Solution:Purity (HPLC): ≥ 99% Concentration: 100mM ± 5%pH: 7.0 ± 0.1Lambda Max: 272nm ± 2nmExtinction Coefficient: 9300 (pH 7, 20mM potassium phosphate) A250/A260: 0.80 ± 0.03A280/A260: 0.96 ± 0.03DNase: None DetectedRNase: None Detected
• D3189:2’-Deoxyguanosine-5’-Triphosphate, Sodium Salt (dGTP) Solution:Purity (HPLC): ≥ 99% Concentration: 100mM ± 5%pH: 7.0 ± 0.1Lambda Max: 253nmExtinction Coefficient: 13700 (pH 7, 20mM potassium phosphate) A250/A260: 1.15 ± 0.03A280/A260: 0.67 ± 0.03DNase: None DetectedRNase: None Detected
• Storage and Stability:May be stored at -20°C for short-term only. For long-term storage, aliquot and store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• ™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche.