85-1364-76　2’-Deoxythymidine-5’-Triphosphate, Sodium Salt (dTTP) Solution, BioGenomics™ 1250ul　CAS No:18423-43-3,Free Acid: 365-08-2　D3205

特徴

• PCR Grade nucleotides are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
• Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.
• dTTP, Molecular Biology Grade, is a 100mM clear, colorless solution of the sodium salt (pH 8.3).
• Usage:250ul at 100mM = 6,250 standard reactions1,250ul at 100mM = 31,250 standard reactions
• dNDP:≤0.9%
• pH:8.3
• Quality Control:Functionally tested with Sequenase, Taq DNA Polymerase, T7 DNA Polymerase and Pfu DNA Polymerase.
• Concentration:100mM
• Lambda Max:267±2nm
• A250/A260:0.65 ± 0.03
• A280/A260:0.73 ± 0.03
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• ™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche.