85-1361-13　DNA Marker PhiX174, BsuRI (HaeIII), 11 Fragments (72-1353bp), BioGenomics™ 50ug　D3930-35

特徴

• PhiX174 DNA was completely digested by BsuRI, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 11 discrete fragments (in base pairs): 1353, 1078, 872, 603, 310, 281, 271, 234, 194, 118, 72.
• 6x Loading Dye Solution (L3350):Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60mM EDTA, 60% glycerol.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Recommendations for Use:I. Loading on Agarose Gel1. Prepare DNA Marker before loading:1ul (0.5ug) of D3930-35 DNA Marker1ul of L3350 6X Loading Dye Solution4ul of deionized water 2. Vortex gently just prior to use. Do not heat before loading
• 3. Apply the prepared amount (6ul) of the DNA Marker on a 5mm lane of agarose gel
• 4. Following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining
• Use 0.1ug (0.2ul) of the DNA Marker (before dilution) per 1mm of an agarose gel lane width.
• II. Loading on Polyacrylamide Gel1. Prepare DNA Marker before loading:2ul (1ug) of D3930-35 DNA Marker0.5ul of L3350 6X Loading Dye Solution0.5ul of deionized water 2. Vortex gently just prior to use. Do not heat before loading
• 3. Apply the prepared amount (3ul) of the DNA Marker on a 5mm lane of polyacrylamide gel
• 4. Following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining.
• Use 0.2ug (0.4ul) of the DNA Marker (before dilution) per 1mm of a Polyacrylamide gel lane width. 310, 281, 271 bp bands migrate anomalously.